Journal:

Article Title: Global Analysis of Host Cell Gene Expression Late during Cytomegalovirus Infection Reveals Extensive Dysregulation of Cell Cycle Gene Expression and Induction of Pseudomitosis Independent of US28 Function †

doi: 10.1128/JVI.78.21.11988-12011.2004

Figure Lengend Snippet: Abnormal mitotic figures in nonnucleated CMV-infected cells. (A to M) Uninfected or CMV-infected HFs stained with an anti-γtub1 MAb (red), an FITC-conjugated MAb to the viral nuclear proteins IE1/IE2 (green) and Hoechst 44432 to detect cellular DNA (blue). (A to C) γtub1 (A) and cellular DNA (B) localization in a representative uninfected mitotic cell and A+B merged image (C); (D to I) γtub1 (D), cellular DNA (E), and IE1/IE2 (G) localization in a representative infected nonnucleated cell; phase-contrast image of the same cells (H), D+E merged image (F), and F+G+H merged image (I). (J and K) IE1/IE2 (J) and cellular DNA (K) localization in a representative infected nonnucleated cell; (L and M) IE1/IE2 (green) and γtub1 (red) localization in representative infected nonnucleated cells. Magnification, ×1,000.

Article Snippet: Cellular DNA was highlighted with Hoechst 44432 (Molecular Probes, Eugene, Oreg.).

Techniques: Infection, Staining

Journal:

Article Title: Global Analysis of Host Cell Gene Expression Late during Cytomegalovirus Infection Reveals Extensive Dysregulation of Cell Cycle Gene Expression and Induction of Pseudomitosis Independent of US28 Function †

doi: 10.1128/JVI.78.21.11988-12011.2004

Figure Lengend Snippet: Dependence of pseudomitosis on viral and cellular DNA synthesis. (A) Percentage of IE1/IE2 antigen-positive pseudomitotic cells at four different times postinfection in three independent experiments. Each bar represents the mean percentage ± the standard deviation. (B) Percentage of IE1/IE2-positive pseudomitotic cells at 72 hpi in infected HF cultures maintained in the presence of medium (•), medium plus 1 mM HU to block cellular DNA synthesis (▪), medium plus 10 mM HU to block both viral and cellular DNA synthesis (⧫), or medium plus PFA to block viral DNA synthesis (▴). Each symbol represents an independent experiment. Horizontal bars indicate mean values for all experiments.

Article Snippet: Cellular DNA was highlighted with Hoechst 44432 (Molecular Probes, Eugene, Oreg.).

Techniques: DNA Synthesis, Standard Deviation, Infection, Blocking Assay

Journal:

Article Title: Global Analysis of Host Cell Gene Expression Late during Cytomegalovirus Infection Reveals Extensive Dysregulation of Cell Cycle Gene Expression and Induction of Pseudomitosis Independent of US28 Function †

doi: 10.1128/JVI.78.21.11988-12011.2004

Figure Lengend Snippet: Progression of CMV replication cycle in pseudomitotic cells. (A to C) ppUL44 (green) and cellular DNA (red) localization in three representative infected pseudomitotic cells; (D) BrdU (green) incorporation and γtub1 (red) localization in a representative infected pseudomitotic cell; (E and F) transmission electron microscopy of a representative infected pseudomitotic cell. The rectangle in panel E shows the area magnified in panel F. Arrows indicate viral DNA encapsidation structures; arrowheads indicate events of partial or complete enclosure of capsids in vesicular structures. Ch, condensed mitotic chromosomes; Mt, mitochondria. Magnification: E, ×3,000; F, ×17,000.

Article Snippet: Cellular DNA was highlighted with Hoechst 44432 (Molecular Probes, Eugene, Oreg.).

Techniques: Infection, Transmission Assay, Electron Microscopy

Journal: Biological Research

Article Title: Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells

doi: 10.1186/s40659-019-0243-6

Figure Lengend Snippet: BBR + DDP combination induced apoptosis. OVCAR3 cells ( a ) and POCCLs ( b ) were treated with BBR (100 μM) and/or DDP (5 mg/L) for 24 h. The gross changes and apoptotic morphology of OVCAR3 cells were detected by Transmission Electron Microscopy (TEM) assay under ×4200 magnification ( a ). The number of OVCAR3 and POCCLs undergoing apoptosis was detected by flow cytometry using Annexin V-FITC/PI double staining ( b ). Data were based on at least three independent experiments, and shown as mean ± SEM. *p < 0.05, **p < 0.01 compared with NC; # p < 0.05, ## p < 0.01 compared with BBR; & p < 0.05, && p < 0.01 compared with DDP

Article Snippet: Cells were treated with BBR (100 μM) or/and DDP (5 mg/L) for 24 h. Indicated cells were digested into single cell suspensions using EDTA-free trypsin and then stained according to the instructions provided with the Annexin V-FITC/PI Apoptosis Detection kit (Beyotime, Shanghai, China).

Techniques: Transmission Assay, Electron Microscopy, Flow Cytometry, Double Staining

Journal: Redox Report : Communications in Free Radical Research

Article Title: Ferroptosis inhibition and mitochondrial rescue: a novel mechanism of emodin in rheumatoid arthritis

doi: 10.1080/13510002.2026.2646383

Figure Lengend Snippet: EMO improves joint histopathological changes and structural damage in CIA mice. (A) EMO's chemical structure. (B) Schematic of the animal experiment. (C) Representative photographs of the hind paws of mice in each group. (D) Representative H&E staining images of ankle joints in each group (200×); red arrow: indicates inflammatory cell infiltration. (E) Representative Safranin O–Fast Green staining images of ankle joints in each group (100×, 200×); blue arrow: indicates cartilage erosion or bone destruction. (F) Representative Micro-CT images of the hind paws of mice in each group; yellow arrows: indicates cartilage erosion or bone destruction. (G–I) Quantitative analyses of Tb.Th, BV/TV and BMD in the hind paw bones of mice (n = 6); data are presented as mean ± standard deviation (SD). ### p < 0.001 vs control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs the model group. Control group: normal mouse group; Model group: CIA mouse group; EMO-L group: low-dose EMO group, 25 mg/kg; EMO-M group: medium-dose EMO group, 50 mg/kg; EMO-H group: high-dose EMO group, 100 mg/kg; MTX group: methotrexate group, 10 mg/kg; H&E: hematoxylin and eosin staining; TEM: transmission electron microscopy; Tb.Th: trabecular thickness; BV/TV: bone volume/total volume; BMD: bone mineral density.

Article Snippet: Specimens of mouse paws were preserved in 4% paraformaldehyde for a duration of 24 h and subsequently examined with a high-resolution micro-CT system (Skyscan1276, Bruker Corporation, USA).

Techniques: Staining, Micro-CT, Standard Deviation, Control, Transmission Assay, Electron Microscopy